应用领域
MasterAmp™超长PCR试剂盒是一个完整的系统,可成功,准确地一步扩增多达40 kb的DNA序列。该试剂盒还包含9个具有dNTP,缓冲液和不同量的MgCl 2 的MasterAmp 超长PCR 2X PreMix,以及含甜菜碱的MasterAmp PCR增强剂。*对每个新模板和引物组执行步骤1(图1)。一个PreMix将扩增长模板,并在所有后续扩增中给出一致的结果(图1,步骤2)。
好处
Applications
The MasterAmp™ Extra-Long PCR Kit is a complete system for successful and accurate one-step amplification of DNA sequences up to ~40 kb. The kit also contains nine MasterAmp Extra-Long PCR 2X PreMixes with dNTPs, buffer, and varying amounts of MgCl2 and the MasterAmp PCR Enhancer with betaine.* Perform Step 1 (Fig. 1) with each new template and primer set. One PreMix will amplify the long template and give consistent results in all subsequent amplifications (Step 2, Fig. 1).
Benefits