lucigen 60215-2 25 rxns (FIVERs) 电感受态细胞
产品简介:北京中北林格供应 lucigen 60215-2 25 rxns (FIVERs) 电感受态细胞,中北林格是lucigen总代理,提供lucigen产品货号报价查询。代理的其他品牌包括Biosearch technologies总代理,epicentre总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。</p>
产品名称:
E. cloni® 10G BAC-Optimized Electrocompetent Cells
lucigen 60215-2 25 rxns (FIVERs) 电感受态细胞
货号:60215-2
规格:25 rxns (FIVERs)
存储温度:-80º C
lucigen 60215-2 25 rxns (FIVERs) 电感受态细胞 产品说明书
BAC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells
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Cells that enable the most challenging library construction and screening.
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Highest available transformation efficiencies for library construction and screening.
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Optimized for high yields of DNA.
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可进行zui具挑战性的文库构建和筛选的细胞。
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图书馆建设和筛选使用的转换效率zui高。
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优化用于高产量的DNA。
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详情:
Lucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:
Lucigen提供了两种用于BAC文库构建或大型构建体转化的细jun菌株:
BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight®Cloning Kits.
BAC优化的Replicator™v2.0电感受态细胞 (> 1×1010 cfu / µg pKanR DNA)。这些细胞是CopyRight®Kit中pSMART®BAC或pEZ™BAC载体按需扩增拷贝数所必需的,从而提高了DNA产量。BAC优化的复制细胞是Lucigen的成分 CopyRight®Cloning套件。
E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning. Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.
E. cloni 10G BAC优化的电感受态细胞 ( > 1×107 cfu / µg 150 kb BAC DNA; > 1×1010 cfu / µg pUC DNA)。Lucigen开发的第①批感受态细胞专门用于大型DNA克隆。10G BAC优化细胞采用新颖的程序制造,可通过非常大的插入片段zui大化转化效率,是BAC文库构建和大型构建体转化的绝加选择。
Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning
E. cloni Cell Lines
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Transformation Efficiency
(cfu/µg pUC or pKanR DNA)
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Cloning Methylated DNA
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BAC, Cosmid Cloning
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Blue/White Screening
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10G BAC-Optimized Electrocompetent
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>1 × 1010
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YES
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YES
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YES
without IPTG induction
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BAC-Optimized Replicator v2.0 Electrocompetent
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>1 × 1010
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YES
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YES
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YES
IPTG induction required
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Contact your local distributor for pricing
ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube) or FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.
订购信息
感受态细胞包括对照DNA和恢复培养基,并按照DUO(每管2个转化)或FIVER(每管5个转化)的形式包装。还可以单独使用恢复介质。除非另有说明,否则指定的转化效率适用于pUC DNA。
请注意: 对于所有Lucigen感受态细胞,散装(比下面zui大的零售包装大10倍)和/或定制包装的价格非常诱人。请联系中北林格。
对此产品有兴趣,可联系中北林格获取更多详细信息。</p>
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