11号染色体开放阅读框74抗体

一抗操作步骤：

洗涤：‌洗涤的目的是为了洗去一抗的非特异性结合，‌洗涤的效果直接影响结果背景的深浅。‌建议使用PBST或TBST作为洗涤液，‌其中的Tween20有助于去除非特异性的结合，‌减少背景。‌洗涤的次数和时间根据具体实验来确定，‌建议清洗3-5次，‌每次5分钟。‌

11号染色体开放阅读框74抗体

英文名称：C11orf74

中文名称：11号染色体开放阅读框74抗体

别    名；Chromosome 11 open reading frame 74; FLJ38678; HEPIS; Hypothetical protein LOC119710; Protein HEPIS; CK074_HUMAN.

研究领域；心血管  细胞生物  神经生物学

抗体来源；Rabbit

克隆类型；Polyclonal

交叉反应；(predicted: Human, Mouse, Rat, )

产品应用；WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量；25kDa

细胞定位；细胞核 细胞浆 细胞外基质 分泌型蛋白

性    状；Liquid

浓    度；1mg/ml

免 疫 原；KLH conjugated synthetic peptide derived from human C11orf74: 111-210/221

亚    型；IgG

纯化方法；affinity purified by Protein A

缓 冲 液；0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍：

C11orf74, also known as FLJ38678, is a 123 amino acid protein that exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 11. With approximately 135 million base pairs and 1,400 genes, chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. The chromosome 11 encoded Atm gene is important for regulation of cell cycle arrest and apoptosis following double strand DNA breaks. Atm mutation leads to the disorder known as ataxia-telangiectasia. The blood disorders Sickle cell anemia and β thalassemia are caused by HBB gene mutations. Wilms' tumors, WAGR syndrome and Denys-Drash syndrome are associated with mutations of the WT1 gene. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are also associated with defects in chromosome 11.

抗体孵育注意事项：‌使用干净的孵育盒子，‌避免油脂等杂物沾到膜上造成非特异信号。‌如果一次性需要检测多个蛋白，‌可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜

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