3-磷酸甘油醛脱氢酶抗体
货号
规格
价格
用途
BJ008567
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
商品属性:
英文名称:GAPDH
中文名称:3-磷酸甘油醛脱氢酶抗体
别 名;Glyceraldehyde-3-phosphate dehydrogenase.
产品类型内参抗体
研究领域;细胞生物
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;Human, Mouse, Rat, Hamster,
产品应用;WB=1:5000-40000 ELISA=1:5000-10000 IHC-P=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;37kDa
细胞定位;细胞核 细胞浆 细胞膜
性 状;Liquid
浓 度;1mg/ml
免 疫 原;Recombinant mouse GAPDH protein: 1-333/333
亚 型;IgG
纯化方法;affinity purified by Protein A
缓 冲 液;0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, thereversible oxidative phosphorylation of glyceraldehyde-3-phosphatein the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
公司正在销售的产品:
JAZF1抗体 JAZF1
纯化线粒体氧化酶活性测定试剂盒
Killin-p53调节复制抑制蛋白抗体 KLLN
大鼠载脂蛋白C-II(APOC2)ELISA
亲环素蛋白ppil4抗体 PPIL4
小鼠鞘氨醇1磷酸酯裂解酶1(SGPL1)elisa检测试剂盒
染色体区域稳定蛋白/核输出蛋白1/染色体区域稳定必需蛋白抗体 Exportin 1
人E选择素(E-Sel)elisa检测试剂盒
ZPLD1蛋白抗体 ZPLD1
大鼠P27蛋白(P27)elisa分析检测试剂盒
钙调神经磷酸酶调节蛋白3抗体(唐氏综合征候选区域蛋白1样蛋白3) Calcipressin 3
组织Akt/PKB激酶总活性定量检测试剂盒(A/B/C)
长链脂肪酸延长酶长ELOVL6抗体 ELOVL6
细胞ANDROGEN RECEPTOR 蛋白表达比色法定量检测试剂盒
原肌球蛋白1抗体 Tropomyosin
胞浆微型RNA(miRNA)电泳法分离试剂盒
感觉神经性耳聋常染色体隐性遗传61抗体 Slc26a5
小鼠胱天蛋白酶5(CASP-5)elisa检测试剂盒
γ1氨基丁酸受体α5/GABRα5抗体 GABRA5
结合态淀粉合成酶(GBSS)测试盒
细胞核受体Rev-Erb beta抗体 NR1D2
人蛋白基因产物9.5(PGP 9.5)elisa检测试剂盒
细胞色素C氧化酶蛋白7A3抗体 COX7A3
通用型HSV2(HERPES SIMPLX2)病毒定性检测试剂盒
跨膜蛋白酶丝氨酸1抗体 Hepsin/TMPRSS1
皮质酮(CORT)含量试剂盒(HPLC)
类固醇受体复合物蛋白FKBP5抗体 FKBP51
大鼠三碘甲腺原氨酸(TT3)elisa检测试剂盒
3-磷酸甘油醛脱氢酶抗体磷酸化抑制基因PTEN抗体 Phospho-PTEN (Ser380 + Thr382 + Thr383) 产品类型磷酸化抗体 FAM188B2蛋白抗体 FAM188B2 研究领域;细胞生物 血管活性肠肽抗体 VIP 研究领域; 心血管 学 神经生物学 信号转导 生长因子和 凋亡相关蛋白TGFb信号抗体 ARTS/SEPT4 研究领域;细胞生物 细胞凋亡 细胞周期蛋白