8号染色体开放阅读框74抗体
货号
规格
价格
用途
BJ004944
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:C8orf74
中文名称:8号染色体开放阅读框74抗体
别 名;C8orf74; CH074_HUMAN; Uncharacterized protein C8orf74.
研究领域;细胞生物
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;(predicted: Human, Mouse, Rat, Dog, Pig, Cow, )
产品应用;WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;34kDa
细胞定位;细胞核 细胞浆
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human C8orf74: 31-130/294
亚 型;IgG
纯化方法;affinity purified by Protein A
缓 冲 液;0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
C8orf74 (chromosome 8 open reading frame 74) is a 294 amino acid protein encoded by a gene that maps to human chromosome 8p23.1. Consisting of nearly 146 million bases, chromosome 8 encodes about 800 genes. Translocation of portions of chromosome 8 with amplifications of the c-Myc gene are found in some leukemias and lymphomas, and are typically associated with a poor prognosis. Portions of chromosome 8 have been linked to schizophrenia and bipolar disorder. Trisomy 8, also known as Warkany syndrome 2, most often results in early miscarriage but is occasionally seen in a mosaic form in surviving patients who suffer to a varying degree from a number of symptoms including retarded mental and motor development, and certain facial and developmental defects. WRN is a DNA helicase encoded by chromosome 8 and shown defective in those with the early aging disorder Werner syndrome. Chromosome 8 is also associated with Pfeiffer syndrome, congenital hypothyroidism and Waardenburg syndrome.
一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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