ANGEL2蛋白抗体
货号
规格
价格
用途
BJ004222
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:ANGEL2
中文名称:ANGEL2蛋白抗体
别 名;NGE2_HUMAN; Angel homolog 2; angel2; Protein angel homolog 2.
研究领域; 细胞生物 神经生物学
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;(predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, )
产品应用;WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;62kDa
细胞定位;细胞核
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human ANGEL2: 61-160/544
亚 型;IgG
纯化方法;affinity purified by Protein A
缓 冲 液;0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
ANGEL2 is a 544 amino acid protein that belongs to the CCR4/nocturin family and exists as two alternatively spliced isoforms. The CCR4 family of proteins are 3′–5′-deadenylases that function in the first step of the degradation of poly(A) mRNA. The CCR4 family most likely displays both RNA and ssDNA substrate preferences, thereby implicating a potential role in many regulatory processes. The ANGEL2 gene maps to human chromosome 1 (1q32.3), which is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. Chromosome 1 contains about 3,000 genes, and considering the great number of genes there are also a large number of diseases associated with it. The MUTYH gene is located on chromosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons disease, Gaucher disease and Usher syndrome are also associated with chromosome 1.
一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
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