ATP结合转运因子2抗体
货号
规格
价格
用途
BJ013604
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:TAP2
中文名称:ATP结合转运因子2抗体
别 名;ABC18; ABCB3; Peptide supply factor 2; Peptide transporter PSF2; Peptide transporter TAP2; PSF2; RING 11; TAP 2; Transporter 2 ATP binding cassette sub family B.
研究领域; 细胞生物 信号转导 转录调节因子
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, )
产品应用;ELISA=1:5000-10000 Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;75kDa
细胞定位;细胞膜
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human TAP2: 451-550/686
亚 型;IgG
纯化方法;affinity purified by Protein A
缓 冲 液;0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
产品介绍:
TAP is an integral transmembrane protein involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It is a heterodimer of TAP1 and TAP2, and the peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2. TAP is inducible by interferon gamma and belongs to the ABC transporter family, MDR subfamily. TAP also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. TAP is inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. It is inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP and prevents the conformational rearrangement of TAP induced by peptide binding.
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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ATP结合转运因子2抗体单氨氧化酶A+B抗体 Monoamine oxidase A+B 研究领域;细胞生物 神经生物学 信号转导 锌指蛋白ZBTB7C抗体 ZBTB7C 研究领域;转录调节因子 锌指蛋白 表观遗传学 颅面部发育蛋白1抗体 CFDP1 研究领域;发育生物学 干细胞 FITC标记人CD107a单克隆抗体 human CD107b/FITC 抗体来源;Mouse