CD3抗体

一抗操作步骤：

洗涤：‌洗涤的目的是为了洗去一抗的非特异性结合，‌洗涤的效果直接影响结果背景的深浅。‌建议使用PBST或TBST作为洗涤液，‌其中的Tween20有助于去除非特异性的结合，‌减少背景。‌洗涤的次数和时间根据具体实验来确定，‌建议清洗3-5次，‌每次5分钟。

CD3抗体

英文名称：CD3

中文名称：CD3抗体

别    名；CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide (TiT3 complex); CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; CD3e molecule, epsilon (CD3 TCR complex); CD3e molecule, epsilon (CD3-TCR complex); CD3E_PIG; IMD18; T cell antigen receptor complex epsilon subunit of T3; T cell surface antigen T3/Leu 4 epsilon chain; T cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE.

研究领域；  细胞生物  糖蛋白  t-细胞

抗体来源；Rabbit

克隆类型；Polyclonal

交叉反应；(predicted: Pig, )

产品应用；ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量；20kDa

细胞定位；细胞膜

性    状；Liquid

浓    度；1mg/ml

免 疫 原；KLH conjugated synthetic peptide derived from pig CD3: 11-100/196 <Extracellular>

亚    型；IgG

纯化方法；affinity purified by Protein A

缓 冲 液；0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍：

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008].

抗体孵育注意事项：‌使用干净的孵育盒子，‌避免油脂等杂物沾到膜上造成非特异信号。‌如果一次性需要检测多个蛋白，‌可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜

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