双特异性抗体实验流程 ：
商品介绍：
产品名称 CD45RO抗体 货号 BJ006658 英文名称 CD45RO 浓 度 1mg/ml 研究领域 细胞生物 神经生物学 干细胞 细胞膜受体 细胞表面分子 糖蛋白 细胞类型标志物 自然杀伤细胞 细胞 t-细胞 b-细胞 细胞定位 细胞膜 抗体来源 Rabbit 性 状 Liquid 克隆类型 Polyclonal 亚 型 IgG 理论分子量 180kDa 用途 仅供科研研究实验
产品名称
CD45RO抗体
货号
BJ006658
英文名称
CD45RO
浓 度
1mg/ml
研究领域
细胞生物 神经生物学 干细胞 细胞膜受体 细胞表面分子 糖蛋白 细胞类型标志物 自然杀伤细胞 细胞 t-细胞 b-细胞
细胞定位
细胞膜
抗体来源
Rabbit
性 状
Liquid
克隆类型
Polyclonal
亚 型
IgG
理论分子量
180kDa
用途
仅供科研研究实验
别 名 B220; CD 45; CD45; cd45 antigen; ec3.1.3.48; CD45R; GP180; GP180; GP 180; L CA; LCA; L-CA; Leukocyte common antigen; LY5; Protein tyrosine phosphatase receptor type C; Protein tyrosine phosphatase receptor type c polypeptide; protein tyrosine phosphatase, receptor type, C; Receptor-type tyrosine-protein phosphatase C; PTPRC; PTPRC_HUMAN; SCID due to PTPRC deficiency; T200; T200 glycoprotein; T200 leukocyte common antigen; Human homolog of severe combined immunodeficiency due to PTPRC deficiency. 交叉反应；Human, (predicted: Mouse, Rat, ) 产品应用；ELISA=1:5000-10000 IHC-P=1:100-500 Flow-Cyt=2μg /test （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 免 疫 原；Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.: 21-87/87 亚 型；IgG 纯化方法；affinity purified by Protein A 缓 冲 液；0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD45RO抗体产品介绍： CD45RO is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45% of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies. It is also found on a subset of B cells and on exceptional B cell lymphomas. CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
别 名 B220; CD 45; CD45; cd45 antigen; ec3.1.3.48; CD45R; GP180; GP180; GP 180; L CA; LCA; L-CA; Leukocyte common antigen; LY5; Protein tyrosine phosphatase receptor type C; Protein tyrosine phosphatase receptor type c polypeptide; protein tyrosine phosphatase, receptor type, C; Receptor-type tyrosine-protein phosphatase C; PTPRC; PTPRC_HUMAN; SCID due to PTPRC deficiency; T200; T200 glycoprotein; T200 leukocyte common antigen; Human homolog of severe combined immunodeficiency due to PTPRC deficiency.
交叉反应；Human, (predicted: Mouse, Rat, )
产品应用；ELISA=1:5000-10000 IHC-P=1:100-500 Flow-Cyt=2μg /test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
免 疫 原；Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.: 21-87/87
亚 型；IgG
纯化方法；affinity purified by Protein A
缓 冲 液；0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD45RO抗体产品介绍：
CD45RO is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45% of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies. It is also found on a subset of B cells and on exceptional B cell lymphomas.
CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
单克隆抗体和多克隆抗体都是我们实验中常用的抗体产品。他们在识别的抗原表位、生产和应用等方面有一些区别。 ✓多克隆抗体（polyclonal antibody, pAb）：用一种包含多种抗原决定簇的抗原动物，可刺激机体多个 B 细胞克隆产生针对多种抗原表位的抗体。 ✓单克隆抗体（monoclonal antibodies, mAb）：由单一 B 细胞克隆产生的识别一种抗原表位的抗体。
公司正在出售的产品：
丝裂原活化蛋白激酶MAP4K4重组兔单克隆抗体 MAP4K4 细胞GSK3α激酶活性定量检测试剂盒（A/B/C） 肽基脯氨酰顺反异构酶FKBP10抗体 FKBP10 人胱抑素SN(CST1)elisa分析检测试剂盒 PE标记小鼠CD30单克隆抗体 mouse CD30/PE 细胞JNK/SAPK蛋白表达比色法定量检测试剂盒 Rabbit Anti-Acetyl-Histone H3 (Lys4) antibody 小鼠氨基己糖苷酶Bβ(HEXβ)elisa检测试剂盒 嗅觉受体家族2亚基4抗体 OR2A4 大鼠脂质运载蛋白1(LCN1)elisa检测试剂盒 1号染色体开放阅读框177抗体 C1orf177 仓鼠促卵泡素(FSH)elisa检测试剂盒 鸡毒支原体抗体 MG 酒类激���法定量检测试剂盒 肌动蛋白结合蛋白6抗体 CORO6 大鼠三碘甲状腺原氨酸(T3)elisa检测试剂盒 2-二醇脱氢酶抗体 DHDH 细胞内氧化应激活性氧（ROS）比色法测定试剂盒（单线态氧气） 血小板源性生长因子受体B/PDGFRβ抗体 PDGF Receptor beta 豚鼠干扰素α(IFNα)elisa检测试剂盒 蛋白酪氨酸磷酸酶受体PTPRB抗体 PTPRB 小鼠核糖核酸酶A3(RNASE3)elisa检测试剂盒 癫痫样相关蛋白6抗体 SEZ6L 大鼠白介素6(IL－6)elisa检测试剂盒 F-box蛋白38抗体 FBXO38 冰冻切片肌腺苷酸脱氨酶（Myoadenylate deaminase）活性 FITC标记人CD63单克隆抗体 human CD63/FITC 人β葡萄糖苷酶(β-glucosidase)elisa分析检测试剂盒 CD45RO抗体软骨凝集蛋白CHODL抗体 CHODL 研究领域；细胞生物 发育生物学 NAGPA蛋白抗体 NAGPA 研究领域；细胞生物 神经生物学 信号转导 转录因子SOX-11蛋白重组兔单克隆抗体 SOX11 研究领域； 细胞生物 神经生物学 信号转导 干细胞 转录调节因子 表观遗传学 黑色素瘤缺失样蛋白1抗体 AIM1L 研究领域； 细胞生物
丝裂原活化蛋白激酶MAP4K4重组兔单克隆抗体 MAP4K4
细胞GSK3α激酶活性定量检测试剂盒（A/B/C）
肽基脯氨酰顺反异构酶FKBP10抗体 FKBP10
人胱抑素SN(CST1)elisa分析检测试剂盒
PE标记小鼠CD30单克隆抗体 mouse CD30/PE
细胞JNK/SAPK蛋白表达比色法定量检测试剂盒
Rabbit Anti-Acetyl-Histone H3 (Lys4) antibody
小鼠氨基己糖苷酶Bβ(HEXβ)elisa检测试剂盒
嗅觉受体家族2亚基4抗体 OR2A4
大鼠脂质运载蛋白1(LCN1)elisa检测试剂盒
1号染色体开放阅读框177抗体 C1orf177
仓鼠促卵泡素(FSH)elisa检测试剂盒
鸡毒支原体抗体 MG
酒类激���法定量检测试剂盒
肌动蛋白结合蛋白6抗体 CORO6
大鼠三碘甲状腺原氨酸(T3)elisa检测试剂盒
2-二醇脱氢酶抗体 DHDH
细胞内氧化应激活性氧（ROS）比色法测定试剂盒（单线态氧气）
血小板源性生长因子受体B/PDGFRβ抗体 PDGF Receptor beta
豚鼠干扰素α(IFNα)elisa检测试剂盒
蛋白酪氨酸磷酸酶受体PTPRB抗体 PTPRB
小鼠核糖核酸酶A3(RNASE3)elisa检测试剂盒
癫痫样相关蛋白6抗体 SEZ6L
大鼠白介素6(IL－6)elisa检测试剂盒
F-box蛋白38抗体 FBXO38
冰冻切片肌腺苷酸脱氨酶（Myoadenylate deaminase）活性
FITC标记人CD63单克隆抗体 human CD63/FITC
人β葡萄糖苷酶(β-glucosidase)elisa分析检测试剂盒
CD45RO抗体软骨凝集蛋白CHODL抗体 CHODL 研究领域；细胞生物 发育生物学 NAGPA蛋白抗体 NAGPA 研究领域；细胞生物 神经生物学 信号转导 转录因子SOX-11蛋白重组兔单克隆抗体 SOX11 研究领域； 细胞生物 神经生物学 信号转导 干细胞 转录调节因子 表观遗传学 黑色素瘤缺失样蛋白1抗体 AIM1L 研究领域； 细胞生物
一抗是一种球蛋白，能特异性结合特定蛋白或重点研究的其他生物分子，目的是对其进行纯化、检测和定量。使用小鼠、大鼠、兔子、山羊和其他动物作为宿主，可获得多克隆或单克隆抗体。