C-肽抗体

一抗操作步骤：

洗涤：‌洗涤的目的是为了洗去一抗的非特异性结合，‌洗涤的效果直接影响结果背景的深浅。‌建议使用PBST或TBST作为洗涤液，‌其中的Tween20有助于去除非特异性的结合，‌减少背景。‌洗涤的次数和时间根据具体实验来确定，‌建议清洗3-5次，‌每次5分钟。

C-肽抗体

英文名称：C Peptide

中文名称：C-肽抗体

别    名；proinsulin precursor; Hyperproinsulinemia; INS; Insulin Precursor; IRDN; Proinsulin; Propeptide; C-Peptide; INS_HUMAN.

研究领域；  细胞生物  神经生物学  信号转导  生长因子和  糖尿病  细胞因子  新陈代谢

抗体来源；Rabbit

克隆类型；Polyclonal

交叉反应； (predicted: Human, )

产品应用；ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量；3 kDa

细胞定位；分泌型蛋白

性    状；Liquid

浓    度；1mg/ml

免 疫 原；KLH conjugated synthetic peptide of human C Peptide: 57-87/110

亚    型；IgG

纯化方法；affinity purified by Protein A

缓 冲 液；0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍：

C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

抗体孵育注意事项：‌使用干净的孵育盒子，‌避免油脂等杂物沾到膜上造成非特异信号。‌如果一次性需要检测多个蛋白，‌可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜

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