一抗操作步骤：
洗涤：洗涤的目的是为了洗去一抗的非特异性结合，洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液，其中的Tween20有助于去除非特异性的结合，减少背景。洗涤的次数和时间根据具体实验来确定，建议清洗3-5次，每次5分钟。
肽基脯氨酞顺反异构酶Pinl抗体
货号
规格
价格
用途
BJ012674
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称：PIN1
中文名称：肽基脯氨酞顺反异构酶Pinl抗体
别 名；DOD; NIMA interacting 1; Peptidyl prolyl cis trans isomerase NIMA interacting 1; Peptidyl prolyl cis/trans isomerase NIMA interacting; Pin 1; PPIase Pin1; Prolyl isomerase; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1; Protein NIMA interacting 1; Rotamase Pin1; UBL 5; UBL5; PIN1_HUMAN.
研究领域；细胞生物 神经生物学 细胞周期蛋白 细胞分化
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Sheep, )
产品应用；ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量；18kDa
细胞定位；细胞核 细胞浆
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH conjugated synthetic peptide derived from human Pin1: 56-163/163
亚 型；IgG
纯化方法；affinity purified by Protein A
保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Pin1 has been implicated in tau pathologies that underlie Alzheimer's Disease. Pin1 binds to tau phosphorylated specifically on the Thr231-Pro site and induces conformational changes in tau. Such conformational changes can directly restore the ability of phosphorylated Tau to bind microtubules and promote microtubule assembly and/or facilitate tau dephosphorylation. Pin1 expression inversely correlates with the predicted neuronal vulnerability in normally aged brain and also with actual neurofibrillary degeneration in AD brain. Pin1 could be pivotal for maintainance of normal neuronal
产品介绍：
Essential PPIase that regulates mitosis presumably byinteracting with NIMA and attenuating its mitosis-promotingactivity. Displays a preference for an acidic residue N-terminal tothe isomerized proline bond. Catalyzes pSer/Thr-Pro cis/transisomerizations. Down-regulates kinase activity of BTK. Cantransactivate multiple oncogenes and induce centrosomeamplification, chromosome instability and cell transformation.Required for the efficient dephosphorylation and recycling of RAF1after mitogen activation.
抗体孵育注意事项：使用干净的孵育盒子，避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白，可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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肽基脯氨酞顺反异构酶Pinl抗体F2RL3; F2R Like Thrombin/Trypsin Receptor 3; Coagulation Factor II (Thrombin) Receptor-Like 3; Thrombin Receptor-Like 3; PAR-4; Coagulation Factor II Receptor-Like 3; Proteinase-Activated Receptor-4; Proteinase-Activated Receptor 4; Protease-Activated Receptor-4; PAR4_HUMAN;