一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
糖基转移酶28家族1抗体
货号
规格
价格
用途
BJ008501
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:GLT28D1
中文名称:糖基转移酶28家族1抗体
别 名;ALG13; GLT28D1; ALG13_HUMAN; Asparagine linked glycosylation 13 homolog (S. cerevisiae); Asparagine-linked glycosylation 13 homolog; CXorf45; FLJ23018; FLJ31785; GLT28D1; Glycosyltransferase 28 domain containing protein 1; Glycosyltransferase 28 domain-containing protein 1; Hematopoietic stem/progenitor cells protein MDS031; MDS031; MGC12423; UDP N acetylglucosamine transferase subunit ALG13 homolog; UDP-N-acetylglucosamine transferase subunit ALG13 homolog; YGL047W.
研究领域;细胞生物 信号转导 细胞类型标志物
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应; Rat, (predicted: Human, )
产品应用;WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;126kDa
细胞定位;细胞浆 细胞膜
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human GLT28D1/ALG13: 21-120/1137
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Isoform 2 may be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway.
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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糖基转移酶28家族1抗体2ABB_HUMAN; B55 beta; Beta isoform of regulatory subunit B55 protein phosphatase 2; MGC24888; PP2A B Subunit B Alpha Isoform; PP2A B55beta; PP2A PR55B; PP2A subunit B B beta isoform; PP2A subunit B B55 beta isoform; PP2A subunit B isoform B55 beta; PP2A subunit B isoform B55-beta; PP2A subunit B isoform beta; PP2A subunit B isoform PR55 beta; PP2A subunit B isoform PR55-beta; PP2A subunit B isoform R2 beta; PP2A subunit B isoform R2-beta; PP2A subunit B PR55 beta isoform; PP2A subunit B R2 beta isoform; Ppp2r2b; PR2AB beta; PR2AB55 beta; PR2APR55 beta; PR52B; PR55 beta; Protein phosphatase 2 (formerly 2A) regulatory subunit B (PR 52) beta isoform; Protein phosphatase 2 (formerly 2A) regulatory subunit B beta isoform; Protein phosphatase 2 regulatory subunit B; Protein phosphatase 2 regulatory subunit B beta isoform; SCA 12; SCA12; Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B beta isoform; Serine/Threonine Protein Phosphatase 2A 56kDa Regulatory Subunit Alpha Isoform; Serine/threonine protein phosphatase 2A neuronal isoform; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform.