一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
天门冬酰胺连接糖基化11抗体
货号
规格
价格
用途
BJ003794
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:ALG11
中文名称:天门冬酰胺连接糖基化11抗体
别 名;Asparagine-linked glycosylation protein 11 homolog; AI849156; alg11; ALG11_HUMAN; Asparagine-linked glycosylation 11; Asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast); GT8; UTP14C.
研究领域;细胞生物
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;(predicted: Human, Mouse, Rat, Dog, Horse, )
产品应用;ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;56kDa
细胞定位;细胞浆 细胞膜
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human ALG11: 301-385/492
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Involvement in disease:Defects in ALG11 are the cause of congenital disorder of glycosylation type 1P (CDG1P). A multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
公司正在销售的产品:
CKELISAKit
大鼠前列腺环素合成酶(PGIS)elisa检测试剂盒
猴子N端前脑钠素(NT-proBNP)elisa分析检测试剂盒
尿素琼脂平板
NT-proBNP ELISA KIT
磷脂爬行酶3抗体
人肺耐药蛋白(LRP)elisa分析检测试剂盒
PLSCR3
人并殖吸虫抗体(IgM)elisa检测试剂盒
兔基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)elisa分析检测试剂盒
HumanLRPELISAKit
皮肤T细胞虏获趋化因子抗体
血液结构型内皮细胞一氧化氮合成酶(cNOS/NOS3/eNOS)活性比色法定量检测试剂盒
CCL27
大鼠催产素受体(OXTR)elisa检测试剂盒
腺苷单磷酸活化蛋白激酶γ2抗体 Anti-PRKAG2/AMPKγ2
小鼠原肌球调节蛋白3(TMOD3)elisa检测试剂盒
丝氨酸/苏氨酸蛋白激酶18 Anti-PLK4/STK18
白细胞介素1δ抗体
转录因子Tbx3抗体 Anti-TBX3
IL-1 delta/IL36RN
钠碘转运体蛋白8抗体 Anti-SLC5A8
α2巨球蛋白(α-2M)抗体
辣根过氧化物酶标记的驴抗豚鼠IgG H&L
alpha 2 Macroglobulin
Donkey Anti-Guinea Pig IgG H&L / HRP
小鼠组织因子途径抑制物(TFPI)elisa检测试剂盒
尾加压素2相关肽抗体
天门冬酰胺连接糖基化11抗体BMND1; HBM; HGNC:6697; Low density lipoprotein receptor related protein 5; Low density lipoprotein receptor related protein 7; LR3; LRP7; OPPG; OPS; LRP7; Osteoporosis pseudoglioma syndrome; VBCH2; LRP5_HUMAN .