一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
脱氧胞苷脱氨酶蛋白抗体
货号
规格
价格
用途
BJ004475
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:APOBEC3G
中文名称:脱氧胞苷脱氨酶蛋白抗体
别 名;DNA dC->dU-editing enzyme APOBEC-3G; APOBEC-related cytidine deaminase; APOBEC-related protein; ARCD; APOBEC-related protein 9; ARP-9; CEM15; CEM-15; Deoxycytidine deaminase; ABC3G_HUMAN.
研究领域;细胞生物
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应; Human,
产品应用;WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;46kDa
细胞定位;细胞核 细胞浆
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human APOBEC3G: 201-300/384
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome,
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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脱氧胞苷脱氨酶蛋白抗体APEX1_HUMAN; DNA-(apurinic or apyrimidinic site) endonuclease; EC:3.1.11.2; APE; APE1; APEX; APX; HAP1; REF1; APEX nuclease (APEN); Apurinic-apyrimidinic endonuclease 1 (AP endonuclease 1; APE-1); REF-1; Redox factor-1; DNA-(apurinic or apyrimidinic site) endonuclease, mitochondrial;