一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
微管相关蛋白1A抗体
货号
规格
价格
用途
BJ011238
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:MAP1A
中文名称:微管相关蛋白1A抗体
别 名;MAP 1A; MAP-1A; MTAP1A; MAP 1L; MAP1L; MAP1A heavy chain; Map1a; MAP1A_HUMAN; Microtubule associated protein 1 like; Microtubule Associated Protein 1A; Microtubule-associated protein 1A; MTAP1A; Proliferation related protein p80; Proliferation-related protein p80; AI853608; Map1a; moth1; Mtap-1; Mtap1.
研究领域; 细胞生物 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, )
产品应用;IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;326kDa
细胞定位;细胞浆
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human MAP1A heavy chain: 2651-2750/3014
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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