一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
细胞表面趋化因子受体4相关蛋白4抗体
货号
规格
价格
用途
BJ005389
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:CNOT4
中文名称:细胞表面趋化因子受体4相关蛋白4抗体
别 名;CCR4 NOT transcription complex subunit 4; CCR4-associated factor 4; CCR4-NOT transcription complex subunit 4; CLONE243; CNOT 4; Cnot4; CNOT4_HUMAN; E3 ubiquitin-protein ligase CNOT4; NOT 4; NOT4 (negative regulator of transcription 4 yeast) homolog; NOT4; NOT4H; Potential transcriptional repressor NOT4Hp.
研究领域;细胞生物 通道蛋白 表观遗传学
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;(predicted: Human, Mouse, Rat, Pig, Horse, Rabbit, )
产品应用;IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;64kDa
细胞定位;细胞核 细胞浆 分泌型蛋白
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human CNOT4: 21-120/575
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex.
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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细胞表面趋化因子受体4相关蛋白4抗体Androgen receptor N terminal interacting protein; Androgen receptor N-terminal-interacting protein; ARNIP; CH-rich-interacting match with PLAG1; CHIMP; E3 ubiquitin-protein ligase Pirh2; hARNIP; hPirh2; p53 induced protein with a RING H2 domain; p53-induced RING-H2 protein; PIRH2E; PIRH2F; PRO1996; RCHY1; Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase; RING finger and CHY zinc finger domain-containing protein 1; RING finger protein 199; RNF199; ZFP 363; Zinc finger protein 363; ZN363_HUMAN.