细胞连接相关蛋白1抗体
货号
规格
价格
用途
BJ005768
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:CLASP1
中文名称:细胞连接相关蛋白1抗体
别 名;CLAP1_HUMAN; clasp1; CLIP associating protein 1; CLIP associating protein CLASP1; CLIP-associating protein 1; Cytoplasmic linker associated protein 1; Cytoplasmic linker-associated protein 1; hOrbit1; MAST1; Multiple asters 1; Multiple asters homolog 1; Protein Orbit homolog 1.
研究领域;细胞生物 细胞周期蛋白 细胞分化
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;(predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, )
产品应用;WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;169kDa
细胞定位;细胞核 细胞浆
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human CLASP1: 30-130/1538
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Microtubule plus-end tracking protein that promotes thestabilization of dynamic microtubules. Involved in the nucleationof noncentrosomal microtubules originating from the trans-Golginetwork (TGN). Required for the polarization of the cytoplasmicmicrotubule arrays in migrating cells towards the leading edge ofthe cell. May act at the cell cortex to enhance the frequency ofrescue of depolymerizing microtubules by attaching their plus-endsto cortical platforms composed of ERC1 and PHLDB2. This corticalmicrotubule stabilizing activity is regulated at least in part byphosphatidylinositol 3-kinase signaling. Also performs a similarstabilizing function at the kinetochore which is essential for thebipolar alignment of chromosomes on the mitotic spindle.
一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
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