一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
细胞生长抑制基因39蛋白抗体
货号
规格
价格
用途
BJ007942
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:FAM129A
中文名称:细胞生长抑制基因39蛋白抗体
别 名;Cell growth inhibiting gene 39 protein; Protein FAM129A; Protein Niban; NIBAN_HUMAN.
研究领域; 生长因子和 细胞类型标志物
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;Human, (predicted: Mouse, Rat, Chicken, Horse, )
产品应用;WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;103kDa
细胞定位;细胞浆
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human FAM129A/Cell growth inhibiting gene 39 protein: 455-550/928
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Regulates phosphorylation of a number of proteins involved in translation regulation including EIF2A, EIF4EBP1 and RPS6KB1. May be involved in the endoplasmic reticulum stress response.
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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