一抗操作步骤：
洗涤：洗涤的目的是为了洗去一抗的非特异性结合，洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液，其中的Tween20有助于去除非特异性的结合，减少背景。洗涤的次数和时间根据具体实验来确定，建议清洗3-5次，每次5分钟。
细胞死亡活化蛋白抗体
货号
规格
价格
用途
BJ006306
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称：CIDEC
中文名称：细胞死亡活化蛋白抗体
别 名；Cell Death Activator; Cell death activator CIDE-3; Cell Death Inducing DFFA Like Effector C; Cell death inducing DFFA like effector protein C; Cell death-inducing DFFA-like effector protein C; CIDE 3; CIDE3; CIDE C; CIDEC_HUMAN; Fat specific protein 27; Fat-specific protein FSP27 homolog; FLJ20871; FSP27.
研究领域； 心血管 细胞生物 信号转导 细胞凋亡 表观遗传学
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；Human, Mouse, Rat, (predicted: Pig, )
产品应用；WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量；27kDa
细胞定位；细胞核 细胞浆
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH conjugated synthetic peptide derived from human CIDEC: 101-200/238
亚 型；IgG
纯化方法；affinity purified by Protein A
保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍：
May act as a CEBPB coactivator in white adipose tissueto control the expression of a subset of CEBPB downstream targetgenes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH (Bysimilarity). Binds to lipid droplets and regulates theirenlargement, thereby restricting lipolysis and favoring storage. Atfocal contact sites between lipid droplets, promotes directionalnet neutral lipid transfer from the smaller to larger lipiddroplets. The transfer direction may be driven by the internalpressure difference between the contacting lipid droplet pair. Whenoverexpressed in preadipocytes, induces apoptosis or increases cellsusceptibility to apoptosis induced by serum deprivation or TGFBtreatment. As mature adipocytes, that express high CIDEC levels,are quite resistant to apoptotic stimuli, the physiologicalsignificance of its role in apoptosis is unclear.
抗体孵育注意事项：使用干净的孵育盒子，避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白，可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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