一抗操作步骤：
洗涤：洗涤的目的是为了洗去一抗的非特异性结合，洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液，其中的Tween20有助于去除非特异性的结合，减少背景。洗涤的次数和时间根据具体实验来确定，建议清洗3-5次，每次5分钟。
细胞因子信号传导抑制蛋白1抗体
货号
规格
价格
用途
BJ015061
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称：SOCS1
中文名称：细胞因子信号传导抑制蛋白1抗体
别 名；CISH 1; CISH1; Cytokine Inducible SH2 Protein 1; JAB; JAK Binding Protein; Janus kinase binding protein; SOCS 1; SOCS1; SOCS-1; SSI 1; SSI1; STAT Induced STAT Inhibitor 1; Supressor of cytokine Signalling 1; TEC Interacting Protein 3; TIP 3; TIP3; SOCS1_HUMAN.
研究领域；细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Rabbit, )
产品应用；ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量；23kDa
细胞定位；细胞核 细胞浆
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH conjugated synthetic peptide derived from human SOCS1: 1-100/211
亚 型；IgG
纯化方法；affinity purified by Protein A
保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍：
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.
抗体孵育注意事项：使用干净的孵育盒子，避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白，可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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细胞因子信号传导抑制蛋白1抗体CAHM1_HUMAN; Calcium homeostasis modulator 1; Calcium homeostasis modulator protein 1; CALHM1; FAM26C; Family with sequence similarity 26, member C; MGC39514; MGC39617; Protein FAM26C.