一抗操作步骤：
洗涤：洗涤的目的是为了洗去一抗的非特异性结合，洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液，其中的Tween20有助于去除非特异性的结合，减少背景。洗涤的次数和时间根据具体实验来确定，建议清洗3-5次，每次5分钟。
细胞周期G1的相互作用蛋白抗体
货号
规格
价格
用途
BJ010972
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称：MNAT1
中文名称：细胞周期G1的相互作用蛋白抗体
别 名；MNAT 1; CAP35; CDK activating kinase assembly factor MAT1; CDK7/cyclin H assembly factor; Cyclin G1 interacting protein; MAT1; Menage a trois; MNAT 1; p35; p36; RING finger protein 66; RING finger protein MAT1; RNF66; MAT1_HUMAN.
研究领域；细胞生物 信号转导 细胞周期蛋白 激酶和磷酸酶 表观遗传学
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
产品应用；WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量；36kDa
细胞定位；细胞核
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH conjugated synthetic peptide derived from human MNAT1: 51-150/309
亚 型；IgG
纯化方法；affinity purified by Protein A
保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍：
Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
抗体孵育注意事项：使用干净的孵育盒子，避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白，可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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