一抗操作步骤：
洗涤：洗涤的目的是为了洗去一抗的非特异性结合，洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液，其中的Tween20有助于去除非特异性的结合，减少背景。洗涤的次数和时间根据具体实验来确定，建议清洗3-5次，每次5分钟。
细胞周期及凋亡调节蛋白1抗体
货号
规格
价格
用途
BJ007199
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称：DIS
中文名称：细胞周期及凋亡调节蛋白1抗体
别 名；CARP-1; Carp1; CCAR1; CCAR1_HUMAN; Cell cycle and apoptosis regulatory protein 1; cell division cycle and apoptosis regulator 1; Cell division cycle and apoptosis regulator protein 1; Death Inducer with SAP domain; FLJ10590; FLJ10839; FLJ13376; FLJ20734; MGC7730; RP11-437A18.1.
研究领域；细胞生物 细胞凋亡 细胞周期蛋白
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；(predicted: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, )
产品应用；ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量；133kDa
细胞定位；细胞浆
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH conjugated synthetic peptide derived from human DIS/CCAR1: 731-830/1150
亚 型；IgG
纯化方法；affinity purified by Protein A
保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍：
Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation.
抗体孵育注意事项：使用干净的孵育盒子，避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白，可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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细胞周期及凋亡调节蛋白1抗体ABP/ZF; ABPZF; Alu binding protein with zinc finger domain; Calcium channel CaT1; Calcium transport protein 1; CaT L; CaT Like; CaT-L; CaT-like; CaT1; ecac; ECaC2; Epithelial apical membrane calcium transporter/channel CaT1; Epithelial calcium channel 2; Epithelial calcium channel; HSA277909; LP6728; MGC162545; NUDC; Transient receptor potential cation channel subfamily V member 6; TrpV6; TRPV6_HUMAN; ZFAB.