双特异性抗体实验流程 :
商品属性:
产品名称
腺苷单磷酸活化蛋白激酶β1抗体
货号
BJ004326
英文名称
AMPK beta 1/PRKAB1
浓 度
1mg/ml
研究领域
细胞生物 信号转导
细胞定位
细胞核
抗体来源
Rabbit
性 状
Liquid
克隆类型
Polyclonal
亚 型
IgG
理论分子量
30kDa
用途
仅供科研研究实验
商品介绍:
别 名 5 AMP activated protein kinase subunit beta 1; AMPK; AMPK beta 1 chain; AMPKb; HAMPKb; PRKAB1; 5'-AMP-activated protein kinase subunit beta-1; AMP-activated protein kinase beta subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPK beta -1 chain; 5'-AMP-activated protein kinase beta-1 subunit; AMPKb; AMPK subunit beta-1; AAKB1_HUMAN; AMPK b1; AMPK-b1.
交叉反应; Human, Rat, (predicted: Chicken, )
产品应用;ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
免 疫 原;KLH conjugated synthetic peptide derived from human AMPK beta 1 : 2-100/270
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
腺苷单磷酸活化蛋白激酶β1抗体产品介绍:
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
单克隆抗体和多克隆抗体都是我们实验中常用的抗体产品。他们在识别的抗原表位、生产和应用等方面有一些区别。 ✓多克隆抗体(polyclonal antibody, pAb):用一种包含多种抗原决定簇的抗原动物,可刺激机体多个 B 细胞克隆产生针对多种抗原表位的抗体。 ✓单克隆抗体(monoclonal antibodies, mAb):由单一 B 细胞克隆产生的识别一种抗原表位的抗体。
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一抗是一种球蛋白,能特异性结合特定蛋白或重点研究的其他生物分子,目的是对其进行纯化、检测和定量。使用小鼠、大鼠、兔子、山羊和其他动物作为宿主,可获得多克隆或单克隆抗体。