一抗操作步骤：
洗涤：洗涤的目的是为了洗去一抗的非特异性结合，洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液，其中的Tween20有助于去除非特异性的结合，减少背景。洗涤的次数和时间根据具体实验来确定，建议清洗3-5次，每次5分钟。
腺苷酸活化蛋白激酶γ3抗体
货号
规格
价格
用途
BJ003841
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称：AMPK gamma 3
中文名称：腺苷酸活化蛋白激酶γ3抗体
别 名；5 AMP activated protein kinase subunit gamma 3; AMPK gamma 3 chain; AMPK gamma3; AMPKG3; Protein kinase AMP activated gamma 3 non catalytic subunit; AAKG3_HUMAN.
研究领域； 细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；(predicted: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, )
产品应用；ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量；54kDa
细胞定位；细胞核 细胞浆 细胞膜
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH conjugated synthetic peptide derived from human AMPK gamma 3: 151-250/489
亚 型；IgG
纯化方法；affinity purified by Protein A
保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍：
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
抗体孵育注意事项：使用干净的孵育盒子，避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白，可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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