一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
腺苷酸激酶-1抗体
货号
规格
价格
用途
BJ004319
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:Adenylate Kinase 1
中文名称:腺苷酸激酶-1抗体
别 名;AK 1; AK1; AK-1; KAD1_HUMAN; Adenylate kinase isoenzyme 1; Adenylate kinase soluble; ATP AMP transphosphorylase; Myokinase; ATP-AMP transphosphorylase 1.
研究领域; 心血管 信号转导 新陈代谢
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应; (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Goat, Monkey, )
产品应用;ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;21kDa
细胞定位;细胞浆
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human Adenylate Kinase 1: 131-194/194
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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