一抗操作步骤：
洗涤：洗涤的目的是为了洗去一抗的非特异性结合，洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液，其中的Tween20有助于去除非特异性的结合，减少背景。洗涤的次数和时间根据具体实验来确定，建议清洗3-5次，每次5分钟。
心脏和神经嵴衍生蛋白1抗体
货号
规格
价格
用途
BJ009428
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称：HAND1
中文名称：心脏和神经嵴衍生蛋白1抗体
别 名；autonomic nervous system and neural crest derivatives-expressed protein 1; heart; Basic helix loop helix transcription factor HAND1; Class A basic helix-loop-helix protein 27; Extraembryonic tissues; Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1; HAND 1; HAND1; HAND-1; HAND1_HUMAN; Heart and neural crest derivatives expressed 1; Heart and neural crest derivatives expressed protein 1; Heart-and neural crest derivatives-expressed protein 1; Hxt; Thing 1; Thing1.
研究领域；心血管 发育生物学 干细胞 转录调节因子 表观遗传学
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；Human, Mouse, Dog, (predicted: Rat, Chicken, Pig, Cow, Rabbit, Sheep, )
产品应用；WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量；24kDa
细胞定位；细胞核
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH conjugated synthetic peptide derived from human HAND1: 101-200/215
亚 型；IgG
纯化方法；affinity purified by Protein A
保存条件；Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍：
Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the , could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity).
抗体孵育注意事项：使用干净的孵育盒子，避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白，可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
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