血管扩张刺激磷蛋白抗体
货号
规格
价格
用途
BJ014312
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:VASP
中文名称:血管扩张刺激磷蛋白抗体
别 名;Vasodilator stimulated phosphoprotein; Vasodilator-stimulated phosphoprotein; VASP; VASP_HUMAN.
研究领域; 细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;Human, Mouse, Rat, (predicted: Dog, Cow, )
产品应用;WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;40kDa
细胞定位;细胞浆 细胞膜
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human VASP: 251-350/380
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
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