一抗操作步骤:
洗涤:洗涤的目的是为了洗去一抗的非特异性结合,洗涤的效果直接影响结果背景的深浅。建议使用PBST或TBST作为洗涤液,其中的Tween20有助于去除非特异性的结合,减少背景。洗涤的次数和时间根据具体实验来确定,建议清洗3-5次,每次5分钟。
血小板源性生长因子AA+BB抗体
货号
规格
价格
用途
BJ012469
50ul
1380
仅供科研实验
100ul
2380
200ul
3480
英文名称:PDGFAA + PDGFBB
中文名称:血小板源性生长因子AA+BB抗体
别 名;Becaplermin; c sis; c-sis; FLJ12858; PDGF 1; PDGF 2; PDGF A; PDGF A chain; PDGF B; PDGF B chain; PDGF subunit B; PDGF-2; Pdgfb; PDGFB_HUMAN; Platelet derived growth factor A chain precursor; Platelet derived growth factor alpha polypeptide; Platelet derived growth factor B chain precursor; Platelet derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); Platelet derived growth factor beta polypeptide; platelet-derived growth factor 2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Platelet-derived growth factor subunit B; Platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis; SIS; SSV; v sis platelet derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog).
研究领域; 心血管 细胞生物 神经生物学 信号转导 生长因子和
抗体来源;Rabbit
克隆类型;Polyclonal
交叉反应;Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
产品应用;ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量;12kDa
细胞定位;分泌型蛋白
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH conjugated synthetic peptide derived from human PDGFA and PDGFB: 81-180/241
亚 型;IgG
纯化方法;affinity purified by Protein A
保存条件;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍:
Subcellular Location:
Secreted.
抗体孵育注意事项:使用干净的孵育盒子,避免油脂等杂物沾到膜上造成非特异信号。如果一次性需要检测多个蛋白,可以考虑将膜剪开或采用其他技术如抗体洗脱液处理后再次使用膜
公司正在销售的产品:
组织碱性焦磷酸酶(Alkaline Pyrophosphatase)活性比色法定量检测试剂盒
钙结合蛋白样39抗体 CAB39L
PDGF-B蛋白共沉淀分析试剂盒
抑癌基因5抗体 TUSC5
莱克多巴胺(RAC)残留ELISA检测试剂盒(残留)
TTC30A蛋白抗体 TTC30A
核酸蛋白结合反应试剂盒
ZCCHC6蛋白抗体 ZCCHC6
大鼠纤连蛋白(FN)elisa检测试剂盒
原钙粘蛋白γB1抗体 PCDHGB1
小鼠二氢嘧啶脱氢酶(DPYD)elisa检测试剂盒
维甲酸诱导蛋白17抗体 Retinoic acid induced 17
猪Na+/K+-ATP酶(Na/K ATPase)elisa检测试剂盒
细胞分裂周期蛋白5抗体 APC5
鱼类组织汞元素比色法定量检测试剂盒
跨膜蛋白70抗体 TMEM70
小鼠5羟基吲哚乙酸(5-HIAA)elisa检测试剂盒
AF680标记的跨膜蛋白175抗体 TMEM175, Alexa Fluor 680 conjugated
HEAT重复内含蛋白5B蛋白抗体 HEATR5B
卷曲螺旋结构域蛋白54抗体 CCDC54
IQSEC3蛋白抗体 IQSEC3
APC标记小鼠CD11c单克隆抗体 mouse CD11c/APC
配子**素结合蛋白1抗体 GGNBP1
孕/孕酮(PROG)elisa分析检测试剂盒
桥粒糖蛋白1抗体 Desmocollin 1
肉类D-乳酸比色法定量检测试剂盒
富含半胱氨酸分泌蛋白10抗体 CRISP10
猪脑钠素(BNP)elisa检测试剂盒
血小板源性生长因子AA+BB抗体CCAAT box binding transcription factor; CCAAT-box-binding transcription factor; CTF; NF-I/X; NF1-X; NF1A; NF1X; NFI X; NFI-X; NFI/X; NFIX; NFIX_HUMAN; Nuclear factor 1 X type; Nuclear factor 1 X-type; Nuclear factor 1/X; Nuclear factor I/X; TGGCA binding protein; TGGCA-binding protein.