弹性蛋白检测试剂盒
Biocolor Fastin elastin Assay
利用染料结合的方法来定量检测释放到组织培养液或从生物材料中提取的弹性蛋白。
应用范围：样本可来源于细胞培养基和含细胞外基质成分的组织提取物（如弹性软骨、主动脉、皮肤等），可检测可溶性弹性蛋白原和不可溶性弹性蛋白（抽提后以可溶性弹性蛋白多肽的形式检测）。
样本要求：样本体积5-100ul，包含5-75ug的弹性蛋白
实验操作步骤
弹性蛋白的分离：用弹性蛋白沉淀剂处理样本，使弹性蛋白沉淀下来。
弹性蛋白与染料结合：装有沉淀的弹性蛋白管中，加染料和反应液，反应60min以上
弹性蛋白-染料复合物获得：去掉未结合的染料
复合物染料的释放：添加染料释放剂，使弹性蛋白-染料复合物中的染料释放出来
释放出的染料检测：绘制标准曲线，513nm或405nm处定量检测
产品订购
Product Code: F2000 110 assays
Product Code: F4000 440 assays
https://www.chem17.com/st289923/product_18320443.html
http://www.qbiotec.com/SonList-1381476.html
参考
文献
1
Allen, R. A., Wu, W., Yao, M., Dutta, D., Duan, X., Bachman, T. N., Champion, H. C., Stolz, D. B., Robertson, A. M.,
Kim, K., et al. (2014) Nerve regeneration and elastin formation within poly (glycerol sebacate)-based synthetic arterial grafts one-year post-implantation in a rat model. Biomaterials 35, 165–173.
2
Anilkumar, T. V., Vineetha, V. P., Revi, D., Muhamed, J. and Rajan, A. (2014) Biomaterial properties of cholecystderived scaffold recovered by a non-detergent/enzymatic method. Journal of Biomedical Materials Research Part B:
Applied Biomaterials.
3
AUGUSTIN, C., FREI, V., PERRIER, E., HUC, A. and DAMOUR, O. AN IN VITRO SELECTION OF NEW COSMETIC ACTIVE
COMPOUNDS: FROM SCREENING TESTS ON MONOLAYERED FIBROBLAST CULTURE TO EFFICIENCY STUDY ON 3-D DERMAL
EQUIVALENT. AMMONIO LATTATO" ATTIVATO 1.
4
Baiguera, S., Del Gaudio, C., Kuevda, E., Gonfiotti, A., Bianco, A. and Macchiarini, P. (2014) Dynamic decellularization and cross-linking of rat tracheal matrix. Biomaterials 35, 6344–6350.
5
Baiguera, S., Del Gaudio, C., Lucali, E., Kuevda, E., Boieri, M., Mazzanti, B., Bianco, A. and Macchiarini, P. (2014) Electrospun gelatin scaffolds incorporating rat decellularized brain extracellular matrix for neural tissue engineering. Biomaterials 35, 1205–1214.
6Cheheltani, R., McGoverin, C. M., Rao, J., Vorp, D. A., Kiani, M. F. and Pleshko, N. (2014) Fourier transform infrared spectroscopy to quantify collagen and elastin in an in vitro model of extracellular matrix degradation in aorta. Analyst 139, 3039–3047.
7
Chen, M.-H., Hu, C.-K., Chen, P.-R., Chen, Y.-S., Sun, J.-S. and Chen, M.-H. (2014) Dose-dependent regulation of cell proliferation and collagen degradation by estradiol on ligamentum flavum. BMC musculoskeletal disorders 15, 238.
8
Chow, M.-J., Turcotte, R., Lin, C. P. and Zhang, Y. (2014) Arterial Extracellular Matrix: A Mechanobiological Study of the Contributions and Interactions of Elastin and Collagen. Biophysical journal 106, 2684–2692.
9 Gilpin, S. E., Guyette, J. P., Gonzalez, G., Ren, X., Asara, J. M., Mathisen, D. J., Vacanti, J. P. and Ott, H. C. (2014) Perfusion decellularization of human and porcine lungs: Bringing the matrix to clinical scale. The Journal of Heart and Lung Transplantation 33, 298–308.
10
- 温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
- 免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报