Description
The ViPrimePLUS Avian Infectious Bronchitis Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Avian Infectious Bronchitis Virus genome in blood, tracheal swab and tissue sample.
Avian Infectious Bronchitis Virus (IBV) is a single stranded RNA which belongs to group 3 coronavirus. It mainly causes respiratory disease in chickens, while some nephropathogenic IBV strains colonize the kidney causing nephritis. The disease stress has significantly decreased the meat and eggs production and quality, such as eggs with soft shell and watery albumen. Besides, the disease is highly contagious, leading to the morbidity rate of 100% in unvaccinated population. Both live attenuated and inactivated vaccines are available, but with poor cross-protection. Due to the continual emergence of antigenic variants, vaccination is only partially successful. Although many countries share some common antigenic types, IBV strains within a geographic region are unique and distinct. Therefore, early detection is necessary to avoid major economic losses.
ViPrimePLUS Avian Infectious Bronchitis Virus RT-qPCR is intended for research use only.
Kit Component
- IBV Primers and Probe Mix (IBV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Avian samples (blood, tracheal swab, tissue) |
| Gene Target | ORF1a gene region |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV1003 | ViPrimePLUS Avian Infectious Bronchitis Virus RT-qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Avian Infectious Bronchitis Virus RT-qPCR Kit
