Description
The ViPrimePLUS Avian Influenza A Virus subtype (H5) RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Avian Influenza A virus subtype (H5) genome in blood samples.
Influenza virus is a RNA virus which infects birds and mammals. Transmission is via contact with bodily fluid of infected person or animals. Symptoms of influenza disease are often confused with the less severe common cold, such as fever, sore throat, muscle and headaches, cough and fatigue. In persistent infection, it could develop into pneumonia which is nearly fatal to the children and elderly. The 3 types of influenza are influenza A, influenza B and the low epidemiological concern influenza C. Subtypes are termed based on the surface glycoprotein types, i.e. hemagglutinin (H) and neuraminidase (N). In human, type A and B are the common typical seasonal flu, resulting in 300,000 deaths yearly and up to millions in pandemic years. Pandemic usually occurs when the influenza virus acquired ability through mutation and gene reassortment to cross-infect between animals and human. Influenza A consists of only 1 species and it mainly infects birds and some mammals. Examples of major influenza A pandemics are the 2009 H1N1 flu (swine flu) and 2003 H5N1 flu (avian influenza / bird flu) outbreaks, recording more than 50% mortality rate.
ViPrimePLUS Avian Influenza A Virus subtype (H5) RT-qPCR Kit is intended for research use only.
Kit Component
- H5 Primers and Probe Mix (H5 PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Avian samples (blood) |
| Gene Target | H5 encoding gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV1004 | ViPrimePLUS Avian Influenza A Virus subtype (H5) RT-qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Avian Influenza A Virus subtype (H5) RT-qPCR Kit
