Description
The ViPrimePLUS Trypanosoma cruzi qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Trypanosoma cruzi genomes in clinical samples (e.g. blood).
Trypanosoma cruzi is an insect-borne protozoan which infects humans and animals, causing Chagas' disease. It is transmitted to human via the faeces of the triatomine bugs (also known as kissing bug). Currently, there are about 10 million people being infected with the disease where most cases are reported in Latin America. Chagas disease is exhibited in two phases; 1) the acute phase where the parasite circulates in the body, and 2) the chronic phase where the parasite hides in the heart and digestive tissues, causing damage. Symptoms in the acute phase are mild, such as fever, enlarged lymph glands, swelling of an eye, difficulty in breathing and chest pain. However in chronic state, 30% of the cases will develop cardiovascular disease and 10% will lead to digestive tract complications. If early treatment is not provided, death may follow during to heart failure.
ViPrimePLUS Trypanosoma cruzi qPCR Kit is intended for research use only.
Kit Component
- T. cruzi Primers and Probe Mix (T. cruzi PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. blood) |
| Gene Target | 18S ribosomal RNA |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM4018 | ViPrimePLUS Trypanosoma cruzi qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Trypanosoma cruzi qPCR Kit
