Description
The ViPrimePLUS Bluetongue Virus 8 RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Bluetongue Virus 8 genome in blood, spleen and liver tissue samples.
Bluetongue Virus (BTV) is a double stranded RNA virus which causes the infectious but non-contagious disease in ruminants such as sheep, cattle, deer, goats and camels. There are 24 recognized serotypes worldwide, where each prevails in different geographical regions. The primary mode of transmission of the disease is via midges (genus Culicoides). Once the virus enters the host, it tends to reside in the red blood cells and other cells to evade the host immune reaction, prolonging viremia. Cattle are the major reservoir of the infection due to its extended viremia and host preference by midges. In infected sheep and some deer species, the clinical signs include fever, excessive salivation, lameness, depression, dyspnoea, mucopurulent nasal discharge, ulceration and swelling of the tongue, and cyanotic tongue (blue tongue). However, infected cattle and goats usually have inapparent symptoms. Abortion and the birth of abnormal calves or lambs may occur. BTV serotype 8 has been isolated in America, Africa and Europe and causes serious disease in cattle and goats. There are evidences of vertical transmission and sexual transmission via contaminated semen of this serotype but they are not exclusive.
ViPrimePLUS Bluetongue Virus 8 RT-qPCR Kit is intended for research use only.
Kit Component
- BTV-8 Primers and Probe Mix (BTV-8 PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Ruminant samples (e.g. blood, spleen, liver tissue) |
| Gene Target | Outer capsid protein VP2 (L2) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV6007 | ViPrimePLUS Bluetongue Virus 8 RT-qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Bluetongue Virus 8 RT-qPCR Kit
