Description
The ViPrimePLUS Bovine Herpesvirus 1 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Bovine Herpesvirus 1 genome in tracheal swab, genital fluids and semen.
Bovine herpesvirus 1 (BHV-1), a double-stranded DNA virus, is an important cattle pathogen that contributes to the major economic losses in the industry. It is one of the causing agents for the Bovine Respiratory Disease, a severe pneumonia disease. The infection also causes venereal diseases, encephalitis, conjunctivitis, abortion, and systemic infection in neonate calves. There are several virus subtypes, such as BHV-1.1 which is known as the respiratory form, BHV-1.2 as the genital form and BHV-1.3 as the encephalitic form. However, the distinction is not exclusive as there are cases of respiratory infection by BHV-1.2 and intrauterine infection by BHV-1.1. Latent infection is observed in the nervous system, where the viral reactivation promotes virus transmission and recurrent disease occurrence. Many commercially available vaccines using modified live-attenuated virus cause illnesses in young calves due to the immunosuppression property of the virus.
ViPrimePLUS Bovine Herpesvirus 1 qPCR Kit is intended for research use only.
Kit Component
- BHV-1 Primers and Probe Mix (BHV-1 PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Ruminant samples (e.g. tracheal swab, genital fluids, semen) |
| Gene Target | Putative fibronectin binding protein |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV6008 | ViPrimePLUS Bovine Herpesvirus 1 qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Bovine Herpesvirus 1 qPCR Kit
