Description
The ViPrimePLUS Bovine Leukemia Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Bovine Leukemia Virus genome in the blood, preferably the buffy coat and tumour tissue samples.
Bovine Leukemia Virus (BLV) is a double-stranded RNA retrovirus responsible for enzootic bovine leukosis in cattle. Transmission for the virus is via contact with the contaminated blood or milk (rare). The virus infects the B-lymphocytes causing increased white blood cell count in the infected cattle. The cows will suffer from persistent infection and lymphocytosis, where clinical signs are not obvious. Some may subsequently develop lymphoma (tumour) and spreads throughout the body. At this stage, the clinical signs appear dependent on where the tumour grows for example around the stomach and intestine that causes diarrhoea, bloating and ulcer formation. The cows are usually thin and unable to produce enough milk. Tumour in the heart and spinal cord will cause heart failure and progressive paralysis respectively. The beef producers recognize the BLV infection as a major threat to the economy due to the poor production of meat and milk, and the premature culling of cattle with symptoms. Since no vaccine or treatment is available, early diagnosis is important to control the infection so that the farmers could separate or eliminate the infected animals from the herd.
ViPrimePLUS Bovine Leukemia Virus RT-qPCR Kit is intended for research use only.
Kit Component
- BLV Primers and Probe Mix (BLV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Ruminant samples (e.g. blood, tumor tissue) |
| Gene Target | pol gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV6009 | ViPrimePLUS Bovine Leukemia Virus RT-qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Bovine Leukemia Virus RT-qPCR Kit
