Description
The ViPrimePLUS Campylobacter fetus-venerialis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Campylobacter fetus-venerialis genome in genital swab, semen and aborted fetus.
Campylobacter fetus is a Gram negative bacterium that causes abortion in bovine. It consists of two closely related subspecies; 1) C. fetus subsp. venerealis, and 2) C. fetus, which differs epidemiological and in their clinical importance. C. fetus subsp. venerealis is responsible for bovine genital campylobacteriosis, which is associated with endemic abortion, early embryonic death and infertility. It is found in the genital tract and semen, indicating that the pathogen is sexually transmissible. C. fetus subsp. fetus however, causes sporadic abortion and it normally resides in the intestinal tract. Thus, the mode of transmission is usually via ingestion of contaminated food. It is also recognized as a human opportunistic pathogen, infecting pregnant ladies or immunocompromised patients, causing systemic neurological and vascular complications. Vaccination is available for the organisms.
ViPrimePLUS Campylobacter fetus-venerialis qPCR Kit is intended for research use only.
Kit Component
- C.fetus-venerialis Primers and Probe Mix (C.fetus-venerialis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Ruminant samples (e.g. genital swab, semen, aborted fetus) |
| Gene Target | Insertion sequence ISCfe1 |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV6010 | ViPrimePLUS Campylobacter fetus-venerialis qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Campylobacter fetus-venerialis qPCR Kit
