Description
The ViPrimePLUS Capripoxvirus qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Capripoxvirus genome in the saliva, conjunctival secretions, milk, skin lesions and scabs of cattle, sheep and goats.
Capripoxvirus genus comprises of 3 closely related DNA viruses; 1) goat pox virus (GTPV), 2) sheep pox virus (SPPV) and 3) lumpy skin disease virus (LSDV). These viruses cause significance losses in the livestock industries globally. GTPV and SPPV are endemic to Asia and Southern Africa, while LSDV is confined to sub-Saharan Africa. Infection with GTPV and SPPV are characterized by fever, generalised papules or nodules, internal lesions (particularly in the lungs), and death of sheep and goats. They are transmitted via aerosol route. In endemic areas, the mortality rate is usually less than 10%. However, 100% fatality rate can occur in the young ones. Infection with LSDV in cattle brings symptoms such as fever, discharge from the eyes and nose, nodular, necrotic skin lesions, edema of the limbs, and swollen lymph nodes. It is transmitted predominantly via insect vectors. The mortality rate varies between 20% and 85% and most of the recovered animals develop a lifelong immunity.
ViPrimePLUS Capripoxvirus qPCR Kit is intended for research use only.
Kit Component
- Capripoxvirus Primers and Probe Mix (Capripoxvirus PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Ruminant samples (e.g. saliva, conjunctival secretions, milk, skin lesions, scabs) |
| Gene Target | LSDV116 RNA polymerase subunit encoding gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV6012 | ViPrimePLUS Capripoxvirus qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Capripoxvirus qPCR Kit
