Description
The ViPrimePLUS Chlamydophila abortus qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Chlamydophila abortus genome in clinical samples (e.g. vaginal swabs).
Chlamydophila abortus, formerly known as the Chlamydia psittaci immunotype 1, is the causative agent for ovine chlamydiosis (enzootic abortion of ewes). The infection can also be found in livestock such as cows, goats and pigs. Infection may give rise to abortion in the last 2-3 weeks of gestation, birth of stillborn lambs or weak lambs that do not survive pass 48 hours. However, it is not uncommon to observe the birth of healthy lambs as well. There are rarely any signs before the abortion, except for the behavioural change and a vaginal discharge containing large numbers of chlamydial elementary bodies (EBs) may be observed 24–48 hours before abortion. The C. abortus infection often remains latent in the healthy ewes or lambs, until the animals become pregnant, when the pathogen is activated and it invades the placenta and membrane. In males, C. abortus infections may lead to formation of atrophic testes. Vaccines are available in the market currently.
ViPrimePLUS Chlamydophila abortus qPCR Kit is intended for research use only.
Kit Component
- C. abortus Primers and Probe Mix (C. abortus PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Ruminant samples (e.g. vaginal swabs) |
| Gene Target | Outer membrane protein A (ompA) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV6013 | ViPrimePLUS Chlamydophila abortus qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Chlamydophila abortus qPCR Kit
