Description
The ViPrimePLUS Mycoplasma mycoides cluster qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Mycoplasma mycoides cluster genome in ruminant’s blood and bodily fluid samples.
Myroplasma mycoides cluster comprises the 6 closely related ruminant pathogens, which are the Mycoplasma mycoides subsp. Mycoides Small Colony (MmSC), Mycoplasma mycoides subsp. Mycoides Large Colony (MmLC), Mycoplasma capricolum subsp. capripneumoniae (Mcp), Mycoplasma capricolum subsp. capricolum (Mcc), Mycoplasma mycoides subsp. capri (Mmc), and Mycoplasma bovine group 7 (MBG7). Mycoplasma mycoides subsp. mycoides is the causative agent for the contagious bovine pleuropneumonia (CBPP) disease, while the Mycoplasma capricolum subsp. capripneumoniae is responsible for the contagious caprine pleuropneumonia (CCPP) disease. Cattle with CBPP will suffer from respiratory distress, cessation of rumination, anorexia and severe pleuritic pain. Goats with CCPP will develop respiratory disease, mastitis and arthritis with fever. The disease is transmitted through inhalation of infected respiratory droplets.
ViPrimePLUS Mycoplasma mycoides cluster qPCR Kit is intended for research use only.
Kit Component
- M. mycoides cluster Primers and Probe Mix (M. mycoides cluster PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Animal samples (e.g. respiratory fluids) |
| Gene Target | 16S ribosomal gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV6016 | ViPrimePLUS Mycoplasma mycoides cluster qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Mycoplasma mycoides cluster qPCR Kit
