For Research Use Only in the United States. Not for use in diagnostic procedures.
The MicroVue Osteocalcin immunoassay measures intact (de novo) osteocalcin in serum. Intact osteocalcin may be useful as a biochemical indicator of bone turnover.
Osteocalcin (OC) or BGP (bone gla protein) is found exclusively in bone tissue. It is a 5800 molecular weight extrahepatic vitamin K dependent protein produced by osteoblasts. It contains three gamma-carboxyglutamic acid residues which are thought to be involved in calcium ion and hydroxyapatite binding. It accounts for 10%-20% of the noncollagenous protein in bone. While the in vivo function of osteocalcin is unknown, its affinity for bone mineral constituents implies a role in bone formation.
A Highly Specific Assay for Intact OC
- Does not detect carboxy- or aminoterminal peptides
- Requires tertiary structure for antibody recognition
- Detects intact OC, not OC fragments released during osteoclastic resorption
Product Specifications
|
Format
|
ELISA |
|
Test/kit
|
96 wells/plate |
|
Sample type
|
Serum |
Performance Characteristics
Method: Competitive
Analyte: Intact osteocalcin
Specimen: Serum
Specimen Volume: 25 µL
LOD*: 0.45 ng/L
LLOQ*: 2 ng/mL
HLOQ*: 32 ng/L
Precision CVs within-run: 5%-10%
Precision CVs between-run: 5%-10%
Incubation Time: 3.6 Hours
Cross-reactivity: Intact OC 100% Reduced OC 0% N-, C- peptides 0%
*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
About the Assay
Format
96-well microplate with reagents sufficient to test 40 samples in duplicate
Species Reactivity
Rabbit, Pig, Sheep, Goat, Cow, Horse, Cynomolgus Monkey, Human Cell Culture, Guinea Pig
No recognition of Rat, Mouse, or Dog
Assay Steps
Add 25 µL Standards, Controls, and Samples
Add 125 µL anti-Osteocalcin Antibody
Incubate 2 hours at room temperature
Wash 3 times with 1X Wash Buffer
Add 150 µL reconstituted Enzyme Conjugate
Incubate 60 minutes at room temperature
Wash 3 times with 1X Wash Buffer
Add 150 µL Working Substrate Solution
Incubate 35-40 minutes at room temperature
Add 50 µL Stop Solution
Read Optical Density at 405 nm
Data Reduction: 4 Parameter
Ordering Information
| Catalog Number | Description |
|---|---|
|
8002
|
ELISA Kit |
