Rat Osteocalcin ELISA

For Research Use Only. Not for use in diagnostic procedures

Rat osteocalcin, a 50 amino acid peptide, is the major noncollagen protein found in rat bone. It contains three gamma-carboxyglutamic acid (GLA) residues at positions 17, 21, and 24 and is, therefore, also known as bone GLA protein or BGP. The exact biological function of osteocalcin is not known but the three gamma-carboxyglutamic acid residues confer on it a very strong ability to bind to hydroxyapatite and calcium.

The Rat Osteocalcin ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the measurement of osteocalcin in rat serum, plasma or cell culture media. Two different goat polyclonal antibodies to rat osteocalcin have been purified by affinity chromatography. The antibody which recognizes epitopes within the midregion/C-terminal portion of the peptide is biotinylated for capture. The other antibody which recognizes epitopes within the N-terminal region is conjugated with the enzyme horseradish peroxidase (HRP) for detection.