Description
The ViPrimePLUS Human Herpes Virus 6 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Human Herpes Virus 6 genome in clinical samples (e.g. blood, CSF, saliva and transplant sample).
Human Herpes virus (HHV) is classified into 8 subtypes with each of them manifesting different disease implications. Human Herpes virus 6 (HHV6) is the cause of the common roseola infantum in children. It is a T-lymphotropic virus, infecting the CD4+ T-cells. Mode of transmission is via transfer of bodily fluids and transplant organs. There are 2 genetically distinct variants, i.e. HHV6A and HHV6B. Being a common early childhood infection, most children do not exhibit symptoms but minority will develop roseola infantum and febrile-induced seizures. Like any other herpes virus, infection with HHV6 is lifelong with relapses noted in 20-35% of healthy *****s with mild fever or no symptoms. Complications posed to immunocompromised patients and transplant patients are more severe, causing hepatitis, encephalitis, myocarditis and myelosuppression which may lead to death. The viral DNA can be detected by nucleic acid amplification technique during active infection, thus making it a good screening tool.
ViPrimePLUS Human Herpes Virus 6 qPCR Kit is intended for research use only.
Kit Component
- HHV6 Primers and Probe Mix (HHV6 PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. blood, CSF, saliva, transplant sample) |
| Gene Target | BHLF3, the putative dUTPase gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5024 | ViPrimePLUS Human Herpes Virus 6 qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Human Herpes Virus 6 qPCR Kit
