Description
The ViPrimePLUS Human Immunodeficiency Virus type 1 RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Human Immunodeficiency Virus type 1 genome in clinical samples (e.g. blood and serum).
Human immunodeficiency virus is a retrovirus that causes the commonly known AIDS (Acquired Immunodeficiency Syndrome). Mode of transmission is via bodily fluids such as blood, semen, vaginal fluid and breast milk. Therefore, unprotected sex, sharing of needles and neonatal HIV infection contribute to the spread of the disease. The rapid increase of incidences, especially in the Third World countries, has been alarming, claiming more than 25 million lives in the past 30 years and leading the world's infectious diseases list. HIV destroys the human T-cells, the body's second line of defense, thus rendering a weakened immune system in infected patients. AIDS is the most advanced stage of the infection that usually takes 10-15 years to develop. At this stage, patients suffer from secondary infection complications and cancers which will eventually lead to death. HIV1 and HIV2 differ in terms of their origin, with the former exhibited as a more virulent strain than the latter. Majority of HIV infections globally is caused by the easily transmittable HIV1, while HIV2 is largely confined to West Africa.
ViPrimePLUS Human Immunodeficiency Virus type 1 RT-qPCR Kit is intended for research use only.
Kit Component
- HIV1 Primers and Probe Mix (HIV1 PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. blood, serum) |
| Gene Target | Conserved region of the pol gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5027 | ViPrimePLUS Human Immunodeficiency Virus type 1 RT-qPCR Kit | 150 reactions |
Download
Manual
ViPrimePLUS Human Immunodeficiency Virus type 1 RT-qPCR Kit
