Description
The ViPrimePLUS Dobrava-Belgrade Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Dobrava-Belgrade Virus genomes in clinical samples (e.g. serum).
Hantavirus is a rodent hosted virus that causes hemorrhagic fever with renal syndrome (HFRS) by the Old World hantavirus and hantavirus pulmonary syndrome (HPS) by the New World hantavirus in human. Dobrava-Belgrade virus (DOBV) is the most life-threatening Old World hantavirus in European countries with up to a 12% fatality rate. There are 3 genotypes of DOBV, which are hosted by different Apodemus species. The Apodemus flavicollis hosted DOBV-Af causes severe HFRS in southeast Europe, while the Apodemus ponticus associated DOBV-Ap infection resulted in more moderate disease in the southern European part of Russia. The mild-to-moderate infection by the Apodemus agrarius borne strains (DOBV-Aa) however, are more common in the Central and Eastern Europe. Transmission is via inhalation of aerosolized bodily fluids and bites of the infected rodents. Human to human transmission is yet to be reported. The infection can be treated with intravenous antiviral drugs such as ribavirin and symptoms relieved by fluid and electrolyte replacement.
ViPrimePLUS Dobrava-Belgrade Virus RT-qPCR Kit is intended for research use only.
Kit Component
- DOBV Primers and Probe Mix (DOBV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. serum) |
| Gene Target | Dobrava-Belgrade virus (DOBV) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5007 | ViPrimePLUS Dobrava-Belgrade Virus RT-qPCR Kit | 150 reactions |
Download
Manual
ViPrimePLUS Dobrava-Belgrade Virus RT-qPCR Kit
