Description
The ViPrimePLUS Ebola Virus (2014 Outbreak) RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Ebola Virus genomes in clinical samples (e.g. serum).
Ebola virus (EBOV) is one of the four ebolaviruses known to cause severe hemorrhagic fever with high fatality rate. EBOV genome is a single-stranded negative-sense RNA, belonging to the virus family Filoviridae. The natural Ebola virus host appears to be fruit bats and the virus is transmitted to the human population via close contact with blood secretions or other bodily fluids of such infected animals. The virus later spreads via human-to-human transmission through direct contact with infected people’s blood, secretions and/or bodily fluids, and by contact with contaminated surfaces and materials. The disease is considered an emerging contagious disease resulting in a death rate of around 90%. As the incubation period is 2 to 21 days, humans are not infectious until symptoms develop. Symptoms include fever, fatigue, and muscle pain, followed by rash, vomiting, bloody diarrhea and impaired functions of kidney and liver. Although many countries share some common antigenic types, EBOV strains within a geographic region are unique and distinct. Thus, early detection is necessary to avoid major economic losses.
ViPrimePLUS Ebola Virus (2014 Outbreak) RT-qPCR Kit is intended for research use only.
Kit Component
- EBOV Primers and Probe Mix (EBOV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. serum) |
| Gene Target | Nucleoprotein (NP) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5008 | ViPrimePLUS Ebola Virus (2014 Outbreak) RT-qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Ebola Virus (2014 Outbreak) RT-qPCR Kit
