Description
The ViPrimePLUS Enterovirus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Enterovirus genome in clinical samples (e.g. respiratory fluids, faeces and cerebrospinal fluids).
Enterovirus defines a group of 8 subgenera of single-stranded RNA viruses of the Picornaviridae family. There are 5 subgenera of viruses, which infects human, manifesting various neurological, heart and gastrointestinal diseases. Poliovirus causes poliomyelitis that damages the motor neurons, thus resulting in paralysis. This virus has been eradicated in many countries via vaccination program. Coxsackie virus is the common cause of viral heart disease. It was categorized into 2 serotypes, i.e. group A and group B. Group A viruses can cause Hand, Foot and Mouth disease (HFMD) in children, aside from flaccid paralysis of cardiac muscles. Group B however, causes myocarditis, pericarditis, and meningoencephalitis. Enteroviruses also cause HFMD in children, while echoviruses cause acute febrile illness in small children. The mode of transmission of these viruses is via fecal-oral route, respiratory droplets or bodily fluids.
ViPrimePLUS Enterovirus RT-qPCR Kit is intended for research use only.
Kit Component
- Enterovirus Primers and Probe Mix (Enterovirus PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. respiratory fluids, faeces, cerebrospinal fluids) |
| Gene Target | 5’ non coding region of the genome |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM5009 | ViPrimePLUS Enterovirus RT-qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Enterovirus RT-qPCR Kit
