Description
The ViPrimePLUS Salmonella qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of all Salmonella species genomes in clinical (e.g. faeces), food or water samples.
Salmonella spp. are Gram negative bacteria which were divided into 2 species, i.e. S. enterica and S. bongori. They are gut pathogens and most of the time reside in silent in the intestine or gall bladder of many animals, and are intermittently shed in the faeces. Therefore, intake of contaminated food and poor hygiene practices are the main cause of infection in human. Salmonellosis are manifested in two conditions; 1) gastroenteritis which is a non-systemic intestinal infection leading to abdominal cramps, diarrhea, nausea and fever, and 2) enteric typhoid fever which is a systemic infection where pathogens replicates within cells in the reticuloendothelial system.
ViPrimePLUS Salmonella qPCR Kit is intended for research use only.
Kit Component
- Salmonella Primers and Probe Mix (Salmonella PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material |
• Clinical samples (e.g. faeces) • Food samples • Water samples |
| Gene Target | Invasine A (InvA) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2051 | ViPrimePLUS Salmonella qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Salmonella qPCR Kit
