Description
The ViPrimePLUS Shigella qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Shigella genomes in clinical (e.g. faeces), and food samples.
Shigella sp. is a group of Gram negative bacteria which causes gastroenteritis, namely Shigellosis. The symptoms of infection usually resolve within 5-7 days. In severe infection, high fever may occur causing seizures in children, less than 2 years old. Transmission is through ingestion of contaminated food. The pathogens invade and destroy the cell lining the large intestine, resulting in ulceration and bloody diarrhoea. Several species are recognized as the common human pathogens, such as S. dysenteriae from Serogroup A, which is potentially epidemic, S. flexneri from Serogroup B, which accounts for many incidences in developing countries, S. boydi from Serogroup C, which is limited to the Indian subcontinent, and S. sonnei from Serogroup D which is more genetically diverge than the previous 3 species and is responsible for 2/3 of the cases in USA.
ViPrimePLUS Shigella qPCR Kit is intended for research use only.
Kit Component
- Shigella Primers and Probe Mix (Shigella PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material |
• Clinical samples (e.g. faeces) • Food samples |
| Gene Target | Virulence plasmid pCP301 (VirA) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2053 | ViPrimePLUS Shigella qPCR Kit | 150 reactions |
Download
Manual
ViPrimePLUS Shigella qPCR Kit
