Description
The ViPrimePLUS Staphylococcus epidermidis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Staphylococcus epidermidis genomes in clinical swab samples of the skin, wound and medical equipment.
Staphylococcus epidermidis is a Gram positive, cocci bacterium. This skin colonizer is a commonly known commensal that is also believed to provide protection to the host skin from being colonized by more virulent pathogens. However in recent years, it is also seen as opportunistic pathogen, especially in nosocomial infections. The ability of the pathogenic strains to form biofilm on inert objects, contributes to its major representation in the frequent causative agent involved with infections of indwelling medical devices, such as peripheral or central intravenous catheters (CVCs). The infection is usually introduced from the skin of the patient during device insertion. Second to S. aureus, S. epidermidis is responsible for many bloodstream infection, central nervous system infection, urinary tract infection, eye infection and endocarditis. Due to the increasing antibiotic resistance as a result of continuous exposure, treatment for the patients has remained a challenge.
ViPrimePLUS Staphylococcus epidermidis qPCR Kit is intended for research use only.
Kit Component
- S. epidermidis Primers and Probe Mix (S. epidermidis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. skin, wound and medical equipment) |
| Gene Target | sodA gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2056 | ViPrimePLUS Staphylococcus epidermidis qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Staphylococcus epidermidis qPCR Kit
