Description
The ViPrimePLUS Escherichia coli O104:H4 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Escherichia coli O104:H4 genomes in faecal, food or water samples.
Escherichia coli (E. coli) refers to a diverse group of Gram negative bacteria that resides in the animal and human intestinal tract. Most of them serve as the normal flora in a healthy intestinal tract but some strains are pathogenic. Mode of transmission is normally through contaminated food and water source. The pathogens are divided into 6 pathotypes, i.e. Enterohemorrhagic E. coli (EHEC), Enterotoxigenic E. coli (ETEC), Enteropathogenic E. coli, (EPEC), Enteroaggregative E. coli (EAEC), Enteroinvasive E. coli (EIEC) and Diffusely adherent E. coli (DAEC). EHEC pathotype, which is also known as Shiga-toxin or Verocytotoxin producing E. coli, is responsible for most of the foodborne outbreaks. E. coli 0104:H4 is a rare EHEC strains or EAEC that has acquired the Shiga toxin gene. The outbreak in 2011 has causes global commotion resulting in enhanced food security monitoring. It causes bloody diarrhea, which is normally self-limiting with low mortality risk if treated properly.
ViPrimePLUS Escerichia coli O104:H4 qPCR Kit is intended for research use only.
Kit Component
- E. coli O104:H4 Primers and Probe Mix (E. coli O104:H4 PPM) O157:H7 PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material |
• Faecal samples • Food samples • Water samples |
| Gene Target | Beta-glucuronidase (uidA) gene, flagelin (fliC) gene , O104 antigen gene cluster, tellurite resistance C (terC) gene and Shiga toxin 2 B subunit (strx2B) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2027 | ViPrimePLUS Escherichia coli O104:H4 qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Escherichia coli O104:H4 qPCR Kit
